Plasmid Preparation:Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.
Article Snippet: All statistical analyses were performed with GraphPad Prism (GraphPad Software, Boston, MA). .. Reagent & Antibody Company Catalog number Rabbit polyclonal anti‐Zic2 Abcam ab150404 Goat polyclonal anti‐Iba1 Abcam ab5076 Rabbit polyclonal anti‐CTMP Cell Signaling Technology 4612s Mouse anti‐S100β (C‐3) Santa Cruz Biotechnology sc‐393919 Rabbit polyclonal anti‐β‐actin Cell Signaling Technology 4967 Donkey anti‐goat IgG conjugated with Alexa Fluor 488 ThermoFisher Scientific A11055 Goat anti‐rabbit IgG‐HRP Santa Cruz Biotechnology sc‐2357 Mouse monoclonal anti‐NeuN Abcam Ab104224 Mouse monoclonal anti‐MAP2 Invitrogen 13‐1500 (M13) Rabbit polyclonal anti‐CTMP (IF) Abcam ab106435 Donkey anti‐rabbit IgG antibody conjugated with Alexa Fluor 594 ThermoFisher Scientific A21207 Donkey anti‐mouse IgG antibody conjugated with Alexa Fluor 488 ThermoFisher Scientific A21202 Hoechst 33342 ThermoFisher Scientific 62249 DAPI Biolegend 422801 Zic2‐ORF cDNA (Rat) plasmid Genecopoeia EX‐Rn12827‐M02 pReceiver‐M02 plasmid Genecopoeia EX‐NEG‐M02 pGL3‐enhancer vector Promega E1771 Renilla luciferase vector pRL‐TK Promega E2241 Lipofectamine LTX ThermoFisher Scientific A12621 CTMP‐ORF Genecopoeia EX_Rn17554‐M56_GS M56‐Vector Genecopoeia EX‐NEG‐M56 EndoFectin Max transfection reagent Genecopoeia EF013 Geneticin ThermoFisher Scientific 11811023 Alexa Fluor 647 donkey anti‐rabbit IgG (H+ L) Invitrogen A31573 Rabbit polyclonal anti‐Ki67 Abcam Ab15580 Cell staining buffer Biolegend 420201 10× RIPA buffer ThermoFisher Scientific 89901 Protease inhibitor cocktail Sigma‐Aldrich P2714 4 of 11 Journal of Cellular Physiology, 2025 10974652, 2025, 1, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/jcp.70007 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025]. ..
Luciferase:Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.
Article Snippet: All statistical analyses were performed with GraphPad Prism (GraphPad Software, Boston, MA). .. Reagent & Antibody Company Catalog number Rabbit polyclonal anti‐Zic2 Abcam ab150404 Goat polyclonal anti‐Iba1 Abcam ab5076 Rabbit polyclonal anti‐CTMP Cell Signaling Technology 4612s Mouse anti‐S100β (C‐3) Santa Cruz Biotechnology sc‐393919 Rabbit polyclonal anti‐β‐actin Cell Signaling Technology 4967 Donkey anti‐goat IgG conjugated with Alexa Fluor 488 ThermoFisher Scientific A11055 Goat anti‐rabbit IgG‐HRP Santa Cruz Biotechnology sc‐2357 Mouse monoclonal anti‐NeuN Abcam Ab104224 Mouse monoclonal anti‐MAP2 Invitrogen 13‐1500 (M13) Rabbit polyclonal anti‐CTMP (IF) Abcam ab106435 Donkey anti‐rabbit IgG antibody conjugated with Alexa Fluor 594 ThermoFisher Scientific A21207 Donkey anti‐mouse IgG antibody conjugated with Alexa Fluor 488 ThermoFisher Scientific A21202 Hoechst 33342 ThermoFisher Scientific 62249 DAPI Biolegend 422801 Zic2‐ORF cDNA (Rat) plasmid Genecopoeia EX‐Rn12827‐M02 pReceiver‐M02 plasmid Genecopoeia EX‐NEG‐M02 pGL3‐enhancer vector Promega E1771 Renilla luciferase vector pRL‐TK Promega E2241 Lipofectamine LTX ThermoFisher Scientific A12621 CTMP‐ORF Genecopoeia EX_Rn17554‐M56_GS M56‐Vector Genecopoeia EX‐NEG‐M56 EndoFectin Max transfection reagent Genecopoeia EF013 Geneticin ThermoFisher Scientific 11811023 Alexa Fluor 647 donkey anti‐rabbit IgG (H+ L) Invitrogen A31573 Rabbit polyclonal anti‐Ki67 Abcam Ab15580 Cell staining buffer Biolegend 420201 10× RIPA buffer ThermoFisher Scientific 89901 Protease inhibitor cocktail Sigma‐Aldrich P2714 4 of 11 Journal of Cellular Physiology, 2025 10974652, 2025, 1, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/jcp.70007 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025]. ..
Transfection:Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.
Article Snippet: All statistical analyses were performed with GraphPad Prism (GraphPad Software, Boston, MA). .. Reagent & Antibody Company Catalog number Rabbit polyclonal anti‐Zic2 Abcam ab150404 Goat polyclonal anti‐Iba1 Abcam ab5076 Rabbit polyclonal anti‐CTMP Cell Signaling Technology 4612s Mouse anti‐S100β (C‐3) Santa Cruz Biotechnology sc‐393919 Rabbit polyclonal anti‐β‐actin Cell Signaling Technology 4967 Donkey anti‐goat IgG conjugated with Alexa Fluor 488 ThermoFisher Scientific A11055 Goat anti‐rabbit IgG‐HRP Santa Cruz Biotechnology sc‐2357 Mouse monoclonal anti‐NeuN Abcam Ab104224 Mouse monoclonal anti‐MAP2 Invitrogen 13‐1500 (M13) Rabbit polyclonal anti‐CTMP (IF) Abcam ab106435 Donkey anti‐rabbit IgG antibody conjugated with Alexa Fluor 594 ThermoFisher Scientific A21207 Donkey anti‐mouse IgG antibody conjugated with Alexa Fluor 488 ThermoFisher Scientific A21202 Hoechst 33342 ThermoFisher Scientific 62249 DAPI Biolegend 422801 Zic2‐ORF cDNA (Rat) plasmid Genecopoeia EX‐Rn12827‐M02 pReceiver‐M02 plasmid Genecopoeia EX‐NEG‐M02 pGL3‐enhancer vector Promega E1771 Renilla luciferase vector pRL‐TK Promega E2241 Lipofectamine LTX ThermoFisher Scientific A12621 CTMP‐ORF Genecopoeia EX_Rn17554‐M56_GS M56‐Vector Genecopoeia EX‐NEG‐M56 EndoFectin Max transfection reagent Genecopoeia EF013 Geneticin ThermoFisher Scientific 11811023 Alexa Fluor 647 donkey anti‐rabbit IgG (H+ L) Invitrogen A31573 Rabbit polyclonal anti‐Ki67 Abcam Ab15580 Cell staining buffer Biolegend 420201 10× RIPA buffer ThermoFisher Scientific 89901 Protease inhibitor cocktail Sigma‐Aldrich P2714 4 of 11 Journal of Cellular Physiology, 2025 10974652, 2025, 1, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/jcp.70007 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025]. ..
Staining:Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.
Article Snippet: All statistical analyses were performed with GraphPad Prism (GraphPad Software, Boston, MA). .. Reagent & Antibody Company Catalog number Rabbit polyclonal anti‐Zic2 Abcam ab150404 Goat polyclonal anti‐Iba1 Abcam ab5076 Rabbit polyclonal anti‐CTMP Cell Signaling Technology 4612s Mouse anti‐S100β (C‐3) Santa Cruz Biotechnology sc‐393919 Rabbit polyclonal anti‐β‐actin Cell Signaling Technology 4967 Donkey anti‐goat IgG conjugated with Alexa Fluor 488 ThermoFisher Scientific A11055 Goat anti‐rabbit IgG‐HRP Santa Cruz Biotechnology sc‐2357 Mouse monoclonal anti‐NeuN Abcam Ab104224 Mouse monoclonal anti‐MAP2 Invitrogen 13‐1500 (M13) Rabbit polyclonal anti‐CTMP (IF) Abcam ab106435 Donkey anti‐rabbit IgG antibody conjugated with Alexa Fluor 594 ThermoFisher Scientific A21207 Donkey anti‐mouse IgG antibody conjugated with Alexa Fluor 488 ThermoFisher Scientific A21202 Hoechst 33342 ThermoFisher Scientific 62249 DAPI Biolegend 422801 Zic2‐ORF cDNA (Rat) plasmid Genecopoeia EX‐Rn12827‐M02 pReceiver‐M02 plasmid Genecopoeia EX‐NEG‐M02 pGL3‐enhancer vector Promega E1771 Renilla luciferase vector pRL‐TK Promega E2241 Lipofectamine LTX ThermoFisher Scientific A12621 CTMP‐ORF Genecopoeia EX_Rn17554‐M56_GS M56‐Vector Genecopoeia EX‐NEG‐M56 EndoFectin Max transfection reagent Genecopoeia EF013 Geneticin ThermoFisher Scientific 11811023 Alexa Fluor 647 donkey anti‐rabbit IgG (H+ L) Invitrogen A31573 Rabbit polyclonal anti‐Ki67 Abcam Ab15580 Cell staining buffer Biolegend 420201 10× RIPA buffer ThermoFisher Scientific 89901 Protease inhibitor cocktail Sigma‐Aldrich P2714 4 of 11 Journal of Cellular Physiology, 2025 10974652, 2025, 1, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/jcp.70007 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025]. ..
Protease Inhibitor:Article Title: Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.
Article Snippet: All statistical analyses were performed with GraphPad Prism (GraphPad Software, Boston, MA). .. Reagent & Antibody Company Catalog number Rabbit polyclonal anti‐Zic2 Abcam ab150404 Goat polyclonal anti‐Iba1 Abcam ab5076 Rabbit polyclonal anti‐CTMP Cell Signaling Technology 4612s Mouse anti‐S100β (C‐3) Santa Cruz Biotechnology sc‐393919 Rabbit polyclonal anti‐β‐actin Cell Signaling Technology 4967 Donkey anti‐goat IgG conjugated with Alexa Fluor 488 ThermoFisher Scientific A11055 Goat anti‐rabbit IgG‐HRP Santa Cruz Biotechnology sc‐2357 Mouse monoclonal anti‐NeuN Abcam Ab104224 Mouse monoclonal anti‐MAP2 Invitrogen 13‐1500 (M13) Rabbit polyclonal anti‐CTMP (IF) Abcam ab106435 Donkey anti‐rabbit IgG antibody conjugated with Alexa Fluor 594 ThermoFisher Scientific A21207 Donkey anti‐mouse IgG antibody conjugated with Alexa Fluor 488 ThermoFisher Scientific A21202 Hoechst 33342 ThermoFisher Scientific 62249 DAPI Biolegend 422801 Zic2‐ORF cDNA (Rat) plasmid Genecopoeia EX‐Rn12827‐M02 pReceiver‐M02 plasmid Genecopoeia EX‐NEG‐M02 pGL3‐enhancer vector Promega E1771 Renilla luciferase vector pRL‐TK Promega E2241 Lipofectamine LTX ThermoFisher Scientific A12621 CTMP‐ORF Genecopoeia EX_Rn17554‐M56_GS M56‐Vector Genecopoeia EX‐NEG‐M56 EndoFectin Max transfection reagent Genecopoeia EF013 Geneticin ThermoFisher Scientific 11811023 Alexa Fluor 647 donkey anti‐rabbit IgG (H+ L) Invitrogen A31573 Rabbit polyclonal anti‐Ki67 Abcam Ab15580 Cell staining buffer Biolegend 420201 10× RIPA buffer ThermoFisher Scientific 89901 Protease inhibitor cocktail Sigma‐Aldrich P2714 4 of 11 Journal of Cellular Physiology, 2025 10974652, 2025, 1, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/jcp.70007 by IN A SP - N E PA L , W iley O nline L ibrary on [02/02/2025]. ..
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